Action for Primates
In our news or take action pages, we report on the types of research to which non-human primates are being subjected. Bear in mind that these reports comprise only a tiny example of the dozens of experiments published each week. See elsewhere for examples from previous years.
Although we emphasise welfare issues with respect to how the research impacted on the individuals, please bear in mind that the non-human primates used were all essentially wild animals, even if bred in captivity. Because of this, the suffering and stress of being in captivity was inherent in every case.
You can find out more information on all grants made by the National Institutes of Health, the largest funding entity in the US, and all their agencies by using the NIH RePORTER. You can search by grant number, investigator, recipient institution and more (see instructions).
In this publicly funded research, common marmosets were subjected to inescapable electric shocks to their feet in an experiment to study post-traumatic stress disorder (PTSD) in people. The work was done at the Biomedical Primate Research Centre (BPRC), Rijswijk, in the Netherlands, and funded by the European Framework Program 7. See our take action alert.
Post-traumatic stress disorder is an anxiety disorder, which can develop after a traumatic, harmful or life-threatening event, such as combat exposure, an accident or physical assault. The study was done purportedly to evaluate the effect of ketamine which is used as an anaesthetic for people and other animals on fear memory, heart rate, cortisol levels and sleep, which are affected in people suffering from PTSD.
Twelve adult female common marmosets were used in the research. They were placed in a
passive avoidance apparatus with
two compartments (dark vs illuminated). Confined in one of the compartments with an electric grid floor, they were subjected to four random inescapable foot-shocks within a 15-minute period.
The marmosets were subjected to highly invasive abdominal surgery to implant a transmitter device to record sleep electroencephalogram (EEG) (recording of the electrical activity of the brain) and the heart rate by electrocardiogram (ECG). Electrode wires for the ECG were tunnelled through the subcutaneous tissue to the chest and fixed to a muscle and those for the EEG were inserted through the subcutaneous tissue to the skull and fixed by a surgical screw and dental cement.
All the marmosets were killed to study the brains.
Dr Nedim Buyukmihci, Emeritus Professor of Veterinary Medicine at the University of California, stated:
Subjecting non-human primates to inescapable foot shocks in an attempt to simulate PTSD in people is a simplistic and very inhumane way of studying this distressing disorder. The general treatment hypothesis could be tested more effectively and humanely in people with PTSD. The BPRC claims it adheres to the 3Rs and
…promotes the use of alternatives to animal experiments where possible and considers their development to be part of its mission. I believe that this study is contradictory to that.
NOTE WELL: as a result of a formal complaint by Action for Primates:This article has been retracted: please see Elsevier Policy on Article Withdrawal (http://www.elsevier.com/locate/withdrawalpolicy).
This article has been retracted at the request of the Editors-in-Chief as the authors were unable to provide documentation of approval for the interinstitutional assurance /vertebrate animal section of the paper by the relevant authority, Public Health Service (PHS) Office of Laboratory Animal Welfare (OLAW) in the time that was provided.
The work was carried out at Charles River in Canada, but involved researchers from Southwest Research Institute in Texas, Emergent BioSolutions in Maryland and the US Department of Health and Human Services. See our take action alert.
It was approved by the Office of Laboratory Animal Welfare/Vertebrate Animal Section (OLAW/VAS) in the US, and publicly funded in whole or in part by the Department of Health and Human Services. It was also approved by the Canadian Council on Animal Care, who claim that they ensure
…humane treatment… of animals.
Cyanide is one of the most lethal poisons known and death can occur within minutes following exposure. According to the authors of the research reported here, the objective was to demonstrate the effectiveness of isoamyl nitrite, a cyanide antidote, against lethal potassium cyanide given intravenously in conscious non-human primates. This is despite the fact that amyl nitrite has been used as a cyanide antidote in people for decades.
Twenty-two rhesus macaques were used (11 females, 11 males). They were anaesthetised and subjected to major surgery to have telemetry devices implanted into the abdominal cavity to monitor cardiovascular and lung functions; a pressure catheter implanted into the femoral artery; electrocardiography (ECG) leads implanted into the jugular vein to the heart and diaphragm; a pressure catheter implanted into the left ventricle of the heart and respiratory function leads implanted inside the animals, near the seventh rib.
Following recovery from this surgery, the conscious macaques were placed on a sling and given potassium cyanide intravenously. Some individuals were then treated with intranasal isoamyl nitrite at different doses and different times. The others were not treated with the isoamyl nitrite. According to the researchers, the
…monkeys were monitored for survival, clinical signs, respiratory and cardiovascular changes via continuous telemetry recording.
Shortly after the injection of potassium cyanide, all monkeys showed clinical signs
…leading to a serious deteriorating condition or death. Within three minutes, the macaques had salivation, drooping of eyelids (ptosis), agitation, decreased activity, skin paleness and vomiting. Within the next 3-4 minutes, they had difficulty breathing, were gasping, their eyes were moving involuntarily (nystagmus), their pupils were dilated, they had involuntary muscle spasms and some were convulsing. For some of the animals, the antidote resulted in an improvement in clinical signs with
…complete recovery within an hour post-dose. Those individuals who were not treated or who were treated with a low dose of isoamyl nitrite, however, continued to deteriorate until they died within 10-15 minutes of being poisoned with potassium cyanide. Two macaques
…survived the challenge, but remained lying on the cage floor without any signs of recovery… and were killed, but not until three or five hours after treatment!
This 'research' was exceptionally appalling and barbaric. It is unquestionable that all the macaques even those treated with the antidote suffered substantially. In many studies in which it is known that the animals will suffer, the researchers develop guidelines to decide at what point the animals should be killed the so-called 'humane endpoint'. The researchers in the present study, however, did not even provide for this situation. Instead, some of these macaques were allowed to die with no relief provided to ease the pain and profound suffering from cyanide poisoning. The only ones who were killed out of concern for 'humaneness' were allowed to suffer for up to five hours first.
Scientists routinely try to justify their use and killing of unquestionably non-consenting and unwilling individuals, such as the rhesus macaques in this 'study', by stating that they only do this when absolutely necessary and only to further medical benefits to people. This study, however, shows unequivocally that such pronouncements are not only untrue, there are also no moral boundaries that some scientists will not cross. Moreover, it is difficult to understand how an agency concerned with humanity Health & Human Services could fund such work. Even more demoralising is that the federal agency whose obligation is to ensure the
humane care and
welfare of non-human animals used in research, funded by US tax payers and comprised of veterinarians Office of Laboratory Animal Welfare could allow such brutal treatment of non-human primates.
Cambridge University has developed a protocol that causes considerable harm to common marmosets (Callithrix jacchus) and is promoting this widely in the scientific community by publishing this 'how to' paper. See our take action alert.
The researchers describe the methodology they use to create a
model of anhedonia-like 'symptoms' and depression in marmosets. Anhedonia is the inability to feel pleasure from things that normally would cause pleasure. Blunted sensitivity to rewards is associated with anhedonia and is a core feature of depression in people. There is no evidence that any other primate species experiences this. Further, because there is no way to know what the marmosets are feeling, the researchers have to limit their claim to measuring or looking for
anhedonia-like [emphasis added] signs in the animals, not
symptoms as they incorrectly state.
To create this
model, the researchers use
Pavlovian conditioning. Once the marmosets are
trained, drugs are then injected into the regions of the brain where activity is relevant to impaired reward processing in depression in people and, the researchers claim, can be used to test the efficacy of antidepressant drugs. The marmosets are being used as living test vehicles for testing drugs for human mental health issues.
The work is being done at the Behavioural and Clinical Neuroscience Institute, University of Cambridge, and financially supported by the Medical Research Council, a publicly funded organisation, and Wellcome Trust, a charitable foundation.
The recently published paper describes the protocol the researchers use, step-by-step, starting with the
training of the marmosets to carry out certain tasks. Food rewards (non-nourishing
marshmallow sweets) are used in
training and testing. The marmosets are tested on five days out of seven each week. In order to
entice the marmosets to work, they are fed a restricted diet on testing days. The marmosets are put into a
Pavlovian discrimination testing chamber an enclosed testing box with LED strips and audio speakers (see photos below). You can click here to see a video from the article, showing a marmoset being
tested in this chamber (be aware that the video is 87 MB and may take some time to download and display).
Marmosets are subjected to at least two major surgeries: one to implant a telemetric monitor into the abdomen via a laparotomy. This involves an incision through the abdominal wall into the abdominal cavity where the aorta is punctured to insert the telemetry probe catheter. The other surgery is to implant intracerebral cannulae to target different areas of the brain with drugs. The anaesthetised marmoset is placed in a stereotaxic frame (see photo) and the head is fixed rigidly in place, using bars in the ears, eyes and mouth. The skin of the head is cut and the skull exposed. A dental drill is used to drill holes through the skull and into the brain cavity. The protective lining of the brain (dura mater) is removed with a needle and cannulae inserted into the brain tissue. A mount for access to the cannulae is attached to the skull using dental adhesive.
Researchers claim that infusion of the drug dihydrokainic acid into a specific area in the marmoset brain results in over-activity in this area sufficient to blunt the anticipatory arousal to reward. They claim this is evidence for the correlative findings in humans that over-activity in this region (identified using functional neuroimaging in people) is associated with blunted reward responsivity seen in anhedonia and depression.
The complex emotional, genetic and environmental stressors that cause mental illness and depression in people cannot be compared with the simplistic marmoset
model created by these researchers. Further, it is clear from the authors own statements that the issues can be and have been studied in people and that there are serious
limitations in the marmoset, including:
…marmosets are sensitive and temperamental animals whose behavior is sensitive to slight changes in their social and physical environment.
…the manipulations described here are all acute intracerebral pharmacological infusions which do not fully mimic the chronic depressed state.
The authors attempt to convince the reader of their humanity towards the marmosets by describing the care they provide:
Paired housing wherever possible [emphasis added] and
A spacious home cage with enrichment objects. The authors also claim that they keep the marmosets
...in a manner that fully supports their biological and social needs. This is incorrect given that marmosets are highly social animals, live in lush jungle habitat with an abundance of trees for climbing and nesting, and an extensive and rich variety of food. None of what the authors provide the marmosets comes even close to approximating a normal environment or providing for a normal lifestyle that ensures the maximum opportunities for well-being.
The University of Cambridge claims
We place good welfare at the centre of all our animal research and aim to meet the highest standards…. It is claimed that their Animal Welfare and Ethical Review Body
…scrutinises research projects and procedures involving the use of live animals to ensure that the 3Rs reduction, refinement and replacement have been adequately applied and retrospectively review research projects with this in mind. This committee is proactive in the promotion of a culture of 3Rs within the University community, ensuring effective experimental design, and promotes awareness of alternatives where available. Action for Primates believes strongly that the marmoset project is completely inconsistent with these claims.
Research involving xenotransplantation the transplantation of living cells, tissues or organs from one species to another is some of the most gruesome work carried out on non-human animals. Such research raises many moral and welfare issues for the individuals who are genetically modified to be used as providers of organs and for those used in the research as recipients of the transplants. Xenotransplantation research involves the major trauma of the transplantation surgery and its inevitable consequences. In addition, the individuals suffer greatly from the immunosuppressive drugs they have to receive to prevent rejection of the transplanted organ, including internal haemorrhage, vomiting, diarrhoea and kidney or heart failure.
The research study reported here was carried out in 2019 and 2020, and took place at the Department of Surgery, University of Alabama in Birmingham. One of the authors is from Revivicor in Virginia, a company involved in regenerative medicine using alternative tissue sources for treatment of human degenerative disease. Revivicor provided two of the pig hearts. Despite this, there is no 'conflict of interest' statement in the publication, something that is normally required. The only financial support referenced in the article is NIH grant P40 OD024628-01 for the institution that provided the baboons (Michael E. Keeling Center). As of 2021-07-05, the amount of public funds awarded through this grant was $1,892,804. See our take action alert.
The authors stated that the mortality rate for human infants on heart transplant wait lists
…remains unacceptably high, and available mechanical circulatory support is suboptimal. The aim of this research was
…to demonstrate the feasibility of utilizing genetically engineered pig (GEP) heart as a bridge to allotransplantation by transplantation of a GEP heart in a baboon.
Four baboons about two years old were subjected to cardiac transplantation, which involves highly invasive chest surgery. Each had their hearts removed and a heart transplanted into them from pigs who were genetically modified. Those baboons who survived the surgery were treated extensively post-operatively to keep them alive and prevent rejection of the hearts.
The fate of the four baboons reveals a picture of substantial suffering and death, with two dying on the day of the surgery (the
B numbers are those assigned to the baboons by the authors):
B217died during the transplant surgery
B37367died while undergoing additional surgery for complications on the same day of the transplant surgery
B15416died unexpectedly on post-operative day 90
B1917died unexpectedly on post-operative day 241
The authors did not provide any details on how the baboons who survived the surgery behaved afterwards, so there was no information on their welfare. Incredibly, even for the two baboons who died
unexpectedly, no information was provided on how they were doing generally prior to death.
The authors made the following statement:
In conclusion, survival of B15416 for 90 days and B1917 for 241 days, demonstrates a significant potential for bridging an infant to allotransplantation with a GEP heart within a decade. The intent, it seems, is to use a pig's heart temporarily in infants while waiting for a human donor.
Action for Primates opposes xenotransplantation research and the 'creation' of genetically engineered non-human animals as living spare-parts 'warehouses' for people. Other options for addressing organ donor shortages should be considered, such as adopting an 'opt-out' approach to organ donations. In this case, unless explicitly expressed otherwise, the deceased is considered to have given tacit approval to be an organ donor.
The US, however, seems intent on adopting xenotransplantation as a solution. Not only does the country operate an opt-in system for donor donation, in 2020, the US Food and Drug Administration (FDA) approved genetically engineered pigs for use in food and medical products. The pigs sold by Revivicor can be used in the production of drugs, to provide organs and tissues for transplants, and to produce meat.
Twelve adult male rhesus macaques were used in this research carried out at Wake Forest School of Medicine, North Carolina, US, which was approved by the Animal Care and Use Committee of Wake Forest University and funded entirely with public funds via National Institute on Drug Abuse (NIDA; a branch of the NIH) grant DA039953. See our take action alert.
The rhesus macaques were used in an attempt to determine whether alcohol causes more sensitivity to cocaine, as if this might answer the same question in people who abuse both drugs. To prepare them for this ordeal, the macaques were subjected to surgery to keep a catheter permanently inside a vein so that they could self-administer the cocaine. They were also fitted with an aluminium collar and restrained in an apparatus euphemistically referred to as a
primate chair, produced by Primate Products (see photo for example of one of their 'primate chairs').
Six of the macaques were coerced to drink ethanol (alcohol). A solution of 4% (w/v) ethanol in 5% Tang® was given to them via an 800-mL drinking bottle attached to each individual's cage for one hour per day, five days per week. By 18 days, the macaques
…drank enough to equal an intake of 2.0 g/kg ethanol during the one-hour period, the equivalent of eight drinks. The researchers called this a
binge-like pattern, and continued this five days a week for about nine months before the start of cocaine self-administration. The other six macaques the
control group were treated exactly the same except that Tang® solution did not contain ethanol.
The cocaine-self administration began with the macaques restrained in the apparatus inside a sound-attenuating chamber. For five mornings per week, they were rewarded with banana-flavoured food pellets if they responded 'correctly' to a specific stimulus. Once a macaque reliably responded and received 30 pellets in a session (which lasted up to an hour), the catheter was implanted. Cocaine was then infused into the bloodstream and testing, in the apparatus, including food rewards, was done to see what effect the cocaine had. The macaques continued to drink an ethanol (alcohol) solution every day after they were returned to their cages.
The researchers did not say anything about the ultimate fate of the macaques, including what happened when the alcohol and cocaine was presumably taken away at the end of the research.
This project is just one more in a continuing series of publicly-funded projects in which non-human primates are forced to consume addictive substances by coercion, in the misguided notion that this will somehow provide answers to substance abuse in people. Drug addiction in people, however, involves considerably more than simply abusing certain substances. The complex combination of factors at play, such as genetics, emotional and personal experiences, and socioeconomic aspects, can never be simulated or resolved through non-human primate research. The millions of tax dollars squandered each year on 'animal models' of drug addition could be better used to directly help the millions of people who suffer from substance abuse.
This experiment was one more in a never-ending attempt to use non-human primates as surrogates for human drug addiction. In this case, it was done in the hopes of developing a squirrel monkey 'model' of economic choice in an attempt to understand drug reinforcement, searching for improved addiction therapies for humans. Economic choice is defined as the behaviour observed when individuals make choices solely based on subjective preferences. In this research, the choice for the squirrel monkeys was between remifentanil, a short-acting opioid drug, and a palatable food reward, diluted sweetened condensed milk.
This work was done at the National Institute on Drug Abuse (NIDA, a branch of the NIH), Baltimore, Maryland, and funded entirely by public funds through NIDA. It was approved by the Institutional Animal Care and Use Committee of the NIDA Intramural Research Program.
Ten adult male common squirrel monkeys (Guianan squirrel monkey) were used, caged individually. They were subjected to experimental sessions carried out over five days per week. During these sessions, they were confined in
custom-built acrylic chairs enclosed within a chamber and facing a touchscreen. A syringe pump was used to provide sweetened condensed milk to a drinking well positioned directly beneath the touchscreen. See photo for schematic.
touchscreen training, the monkeys were rewarded with milk. They had to choose correctly to get a larger reward. Once
trained, the monkeys were anaesthetised and a catheter was surgically implanted in a jugular (neck) or femoral (leg) vein, tunnelled under the skin and exited through the animal's back. An external syringe pump was used to deliver the opioid remifentanil intravenously. After at least one week of recovery, the
training of the monkeys with remifentanil began. The monkeys had to wear
jackets or else they would try to remove these uncomfortable foreign objects implanted into them. Testing involved the touchscreen and the effect of remifentanil on their
success. Trials numbered in the hundreds. The monkeys were also injected with other drugs morphine and naltrexone (an opioid antagonist) to see the impact on their subsequent choice between remifentanil and milk.
No information was provided as to how many weeks or months this experiment continued, the effect of the opioid drug on the monkeys in terms of their general behaviour or well-being or the impact on the monkeys when the remifentanil was taken away. Nor was the ultimate fate of the monkeys stated.
This is yet one more example in a shocking and continuing series of publicly-funded cruel experiments in which non-human primates are forced into addiction research in the misguided notion that this will somehow provide answers and therapies for addiction in people. Drug addiction, however, is a disease in people with no counterpart in any other species. It involves considerably more than simply choosing between an opioid and sweetened milk. The authors of the paper described human economic choice as
A behavioral approach with real-world parallels is one in which the consumption of drugs comes at the expense of alternative nondrug rewards, given that the choice to use drugs can result in the loss of a job, good family relations, and/or good health... They went on to cite human laboratory studies in which
...cocaine users choose sufficiently high monetary rewards over the option to self-administer cocaine , demonstrating that drug users are able to minimize their drug intake when behavioral alternatives are available. It is clear that we already know what is needed to help people, through humane studies in which people can give their informed consent, something not possible with the monkeys!
It is reprehensible that non-human primates who cannot give their consent and are unquestionably unwilling should be manipulated and made to suffer in this kind of 'research'. Human variables such as genetics, emotional and personal experiences, and socioeconomic aspects, can never be simulated or resolved through non-human primate research. The millions of tax dollars spent each year on 'animal models' of drug addiction could be better used to directly help the millions of people who suffer from substance abuse.
When Action for Primates pointed out the welfare and scientific shortcomings of this type of research in a previous letter to NIDA, they, disappointingly, responded with clichéd comments about the issue (personal communication, 24 May 2021):
...animal welfare is of the utmost concern...
Reviewers examine the justification for using animals in each study, whether the research goals can be accomplished using an alternative model...
...committed to ensuring the welfare of animals used in research...
...NIDA will continue to support animal research conducted in accordance with the highest scientific and ethical principles.
The daily use of marijuana is apparently rising in human adolescents, along with the use of high potency marijuana products such as tetrahydrocannabinol (THC). This research subjected black-capped squirrel monkeys to daily injections of cannabis for four months in an attempt to 'understand' the long-term effects of the daily consumption of a high dose of THC in adolescents and whether a therapeutic dose of cannabidiol (CBD) has a modifying effect on the THC. See our take action alert.
Tetrahydrocannabinol is the psychoactive substance that produces the 'high' associated with smoking marijuana and can also lead to central nervous system depression. Cannabidiol is a major component of the active ingredients of marijuana and is used in medical marijuana.
Twelve adolescent male black-capped squirrel monkeys were used in this research carried out at McLean Hospital (part of Harvard University), with collaboration by the University of Toronto. The research was approved by the Institutional Animal Care and Use Committee at McLean Hospital and supported by public funds (NIH grant) and Harvard University.
The monkeys were divided into three groups, one of which was a 'control' group not given the drugs. The remaining monkeys were injected intramuscular daily for four months, receiving an increasing amount of either THC or a combination of THC and CBD, eventually reaching the equivalent of one
marijuana joint equivalent per day.
During the first three weeks, the monkeys underwent
training on a touchscreen. There were
90-training trials during at least five daily sessions. During these sessions, the monkeys were held in a small Plexiglass chamber which was in a sound- and light-attenuating enclosure.
In week four, when the highest dose of drugs was injected, the monkeys were tested using the touchscreen to see the effects of the drugs on their ability to do the task. Some of the monkeys carried out hundreds of trials. The fate of the monkeys was not stated.
The authors concluded:
Whether our observations are relevant to a broader range of cognitive tasks vital for daily function in humans, especially those known to be compromised by marijuana use in human adolescents (i.e., learning and memory, processing speed, complex attention, executive function, impulse control and decision-making) is uncertain. Long term marijuana use initiated during adolescence is associated with a broad spectrum of adverse effects (e.g. cognitive impairment, anxiety, psychosis, amotivation, addiction, accidents) some of which are not identifiable in laboratory housed non-human primates.
As common sense would have foretold, the authors acknowledge that the work subjecting these non-consenting beings to forced captivity, drugs and testing, may have no relevance to people. The authors also concluded that using CBD in addition to THC in the monkeys did not change the outcome.
Drug use is a major public health issue, and there is already a wealth of data on its impact. Testing could easily be carried out using morally-defensible studies in human volunteers and relevant data obtained. Despite this and while the scientific community claims repeatedly that they only use
absolutely necessary, researchers such as these continue to deliberately inflict misery and suffering by forcing drugs into non-human primates. None of these studies, however, can nor could they ever get to the root issues surrounding drug abuse in people, which comprise a complex combination of factors such as genetics, emotional and personal experiences, and socioeconomic issues. While millions of dollars usually paid for by the US tax payer are spent every year to make non-human primates 'drug addicts', people who might actually benefit from these funds and resources continue to suffer.
Rhesus macaques were subjected to an appalling ordeal lasting many months during research to deliberately damage the part of their ear involved in balance. See our take action alert.
The vestibular system (inner ear balance mechanism) is a sensory system that contributes to the sense of balance and spatial orientation for the purpose of coordinating movement. It works with the visual system (eyes, relevant muscles and parts of the brain) to stop objects blurring when the head moves. People with vestibular damage experience impaired vision, spatial perception and balance.
The work was done at the Massachusetts Eye and Ear Infirmary, Boston, Massachusetts (a teaching hospital of Harvard Medical School), and was approved by the Massachusetts Eye and Ear Infirmary Animal Care and Use Committee. It was publicly funded with NIH (NIDCD) grants and privately with a grant from MED EL Corp, a company that produces cochlear implants for hearing issues.
Two female rhesus macaques were used, called
M2 by the authors. They were
…trained to perform a psychophysical task that assayed their perception of head orientation relative to gravity… [authors' emphasis] The 'training', which took six months, was not described, so it is not known whether the monkeys were deprived of food or liquids to 'motivate' them, as usually happens with this type of 'training'.
The monkeys were then subjected to four surgical procedures under anaesthesia:
tunneled subcutaneously to the headcap
Running wires subcutaneously under the skin to the headcap would have involved tearing a path through the tissues beneath the skin, adding to postoperative pain for the monkeys.
The researchers then attempted to carry out a
vestibular ablation, destroying the ability of the monkeys to orient themselves. Monkey
M1 was injected 10 times in each ear with a drug that is toxic. Despite this, the desired loss of function did not occur, leading the researchers to conclude that
Rhesus monkeys appeared to be fairly resistant to this mode of ablation, however, as ten bilateral…injections in M1 only modestly reduced… function. The researchers then tried giving a different drug that is known to be toxic to the ear. They gave two courses of this drug intramuscularly, but this also did not result in the desired loss of function. It was not stated how frequently all these injections were made, whether
M1 was awake or anaesthetised each time and what effects they had on her. Eventually, the researchers gave the original toxic drug directly into a specific part of one ear while implanting electrodes. This was also done in one ear of
M2. In both macaques, the other ear was subjected to a separate surgery:
…the contralateral ear was destroyed with a labyrinthectomy. This resulted in
…complete loss of vestibular function on the non-implanted side and severe damage in the implanted ear…
After subjecting these monkeys to such brutal mutilation, the testing began. The researchers admitted, however, that
Due to delays associated with vestibular ablation and surgical recoveries, testing in the different states was separated by substantial periods of time. As a result, the testing took place over many months. The monkeys had to carry out the task in the dark, immobilised in a restraint apparatus with their heads further immobilised by the head bolt. They had
to rotate a light bar about the roll…axis using a small steering wheel with the goal of aligning the light bar parallel to gravity. Their eye movements were measured. Tilting the chair to see the monkeys' responses was part of the testing process.
There was no information provided on the outcome for the monkeys, their physical condition, their behaviour, how they managed in their cages following such severe disruption to their coordination and orientation. We wrote to the corresponding author of the paper and to the Editor-in-Chief of the journal and asked specifically about the fate of the monkeys. Neither provided us with this information, suggesting that they did not think it was important to include in the publication. We know that damage to a human's vestibular system can cause a person to experience symptoms such as sudden, severe vertigo (spinning/swaying sensation); dizziness; balance problems; lack of coordination; blurred or 'bouncing' vision; hearing abnormalities; anxiety; nausea and vomiting. There is nothing to suggest that monkeys do not experience the same effects. These would unquestionably be extremely frightening to a monkey who cannot understand what is happening to them and who is highly mobile and depends on their agility to survive in their environment.
This research was basic science to learn more 'details' about how balance is controlled in rhesus macaques, but may have no applicability to people. The authors stated that
Considerable work remains before the VI [vestibular implant] can be optimized as a clinical therapy. They also stated that
Human implantation has been initiated by three groups… [authors' emphasis] and that the results are promising. This calls into question, therefore, why macaques were subjected to such an arduous ordeal and mutilation. Despite this, the researchers called for more macaque research.
This experiment carried out on Japanese macaques at Osaka University in Japan, involved using the monkeys as a 'model' for central poststroke pain, which is caused by damage to the brain. The monkeys were deliberately subjected to pain and then the researchers tracked changes in the animals' pain threshold using a behavioural experiment and tracking anatomical and functional changes in the brain by using magnetic resonance imaging (MRI). See our news report.
In two adult male snow monkeys, a specific part of the brain was damaged to create
central poststroke pain (CPSP). Under anaesthesia, part of the scalp was removed and a recording chamber was screwed to the skull with at least one hole into the brain for injecting the compound that would cause the artificial stroke and for making recordings. Head posts were also implanted into the monkeys' skulls. For recordings, the monkeys were forced to sit in a device made of acrylate glass with their lower backs and heads immobilised. Their wrists were also fixed in place to keep their palms facing downwards. An eyeshield was used so that the monkeys could not see their hands and stimulators during the experiment. The monkeys had to do certain tasks and respond to painful stimuli. They were deliberately subjected to pain through a hot stimulus (45-50°C) and a cold stimulus (5-15°C). In their Supplementary Information document, the authors stated that
To avoid burns, each trial took less than 1 minute. … Monkeys could get a food reward if they could endure the stimulus for 1 minute.
The fate of the macaques was not stated.
The authors acknowledged there were major limitations to their research, in design and results. This raises questions as to why the study was approved, subjecting the snow monkeys to this brutal research. Among the limitations listed were 1) the type of artificial brain damage was different to that in people with stroke, 2) the pain induced by the stimulations was not the same as the persistent pain experienced by people suffering from CPSP, and 3) the pain rating scale commonly used for people suffering from CPSP was not used because such
subjective measures could not be obtained from the monkeys. These and the other limitations acknowledged by the researchers reinforce our recurring argument that using non-human primates to study human illnesses is not only morally reprehensible, it also lacks scientific validity.
African green monkeys have lived freely on St Kitts for hundreds of years. Tragically, many of them are exported every year for research primarily to the USA, including individuals captured in the wild. Others are used in research on St Kitts itself. This research took place at the Behavioral Sciences Foundation. See our take action alert.
Eighteen African green monkeys were used in this research to evaluate acellular dermal matrices (ADMs). Acellular dermal matrices are derived from human skin and used in soft tissue reconstructive surgeries as scaffolds to support tissue regeneration. Two already established ADMs were used: SimpliDerm and AlloDerm RTU. Each graft was surgically implanted into nine monkeys. The animals were kept outdoors in individual cages, about 2.5 feet wide, 2.5 feet deep and 3.5 feet high.
During the surgery, an incision was made into the abdomen of the monkeys and all the tissue in the area was removed to create a 3 cm x 7 cm hole into the abdominal cavity. The graft was implanted and the skin and subcutaneous tissue were sutured closed over the wound. Pain relief after the surgery was apparently only provided if a monkey showed
…signs of pain… The authors apparently are unaware that non-human primates, including African green monkeys specifically, may instinctively mask any signs of pain as a means of protecting themselves from predators or to keep social rank, even in a laboratory setting.
Clinical observations were carried out looking for
incisional hernia, hematoma or seroma, as well as any gross abnormalities in general condition, appearance, appetite, stool and urine production, activity level and behavior. No information, however, was provided on the effects of the surgery on the individual monkeys in terms of behaviour, poor health etc. The monkeys were killed at either two, four or 12 week time points and the graft and surrounding tissues were removed from their bodies and examined.
The researchers concluded that the results indicated SimpliDerm was able to promote productive tissue repair and regeneration within three months, and that their work
…may provide important insight into the potential human response to these hADM scaffolds. [emphasis added] But, far from being a definitive study, the researchers stated that
… further studies are necessary to fully realize the clinical utility of this novel hADM. All the researchers are or were employees of Aziyo Biologics, Inc., the producer of the tested commercial product known as SimpliDerm, at the time the work was done.
There was no need to subject the African green monkeys, non-consenting and unwilling beings, to highly invasive surgery, the substantial pain of postoperative recovery and reaction to the wound and implant, and then death. Human acellular dermal matrices are already being used in human patients, as cited by the researchers who used and killed the African green monkeys, and there is at least one ongoing clinical trial sponsored by Aziyo Biologics.
Thirty-four rhesus macaques were used and killed in this recently published research carried out at Boston University School of Medicine in the US. Not only was it approved by their
…Institutional Animal Use and Care Committee (IACUC)…, it was funded entirely by public funds through the National Institutes of Health (NIH). See our take action alert.
The aim of the research was to look at impairments in learning, memory, executive function, and processing speed using the research group's rhesus macaque as a
model of ageing for humans.
The macaques were between five and 30 years old and were used simply to see what changes occurred in their learning ability and their brains with age. They were subjected to a series of behavioural tests to assess learning, memory and executive functions. The testing was carried out five days a week, but for how many hours a day or how many days or weeks was unclear. When the period of testing was completed, the macaques were killed to get their brains, or in the sanitised language of the authors, the
…brains were harvested…. The animals were anaesthetised before being bled to death.
According to the researchers, the macaques exhibit age-related cognitive impairment and can serve as a
model for studying ageing in people. These macaques, however, are essentially wild animals imprisoned in captivity. They will experience severe stresses caused by artificial experimental conditions, something that surely would have an unknown and unmeasurable impact on the their cognitive processes. From a scientific perspective, the findings in the captive macaques would have little, if any, applicability to ageing in people whose lives are fundamentally different and considerably more complex with respect to stressors. The most appropriate species to use to study people is people! More importantly, there are already data on the effects of ageing in people, as acknowledged by the authors, and more can be easily derived from humane and morally defensible studies in people.
This research comprises and exemplifies an appalling waste of life, the work obviously designed just to produce a 'library' of findings to use in future work involving rhesus macaques as a
model. It demonstrates, yet again, that the much-touted refrain of the research community of using 'animals' only when absolutely necessary is just meaningless and disingenuous.
Boston University School of Medicine claims that
The humane care and involvement of animals in research is a serious responsibility shared by the entire research community. In what way was this senseless 'research'
The research was carried out at the University of Fribourg in Switzerland (1). Funding was from several sources, including the European Union's Horizon 2020 research and innovation programme, and the research was approved by the local veterinary authorities of the Canton of Fribourg. The researchers declared the following
Competing interests: three were shareholders and founders of GTX medical, a company producing spinal cord stimulation technologies and who provided part of the research funding, and five were inventors of multiple patent applications and had been granted patents covering parts of this work. See our take action alert.
Five long-tailed macaques were used and were subjected to deliberate spinal cord damage. This involved major surgery to remove parts of their vertebrae to implant spinal electrodes as well as to have muscle electrodes implanted. Electrical stimulation was then applied to parts of their spinal cord to study arm and hand movements. Three of the macaques were used in
terminal procedures and underwent electrophysiological tests. They were killed before waking up from the surgery. The other two macaques were used for
acute procedures in which they were anaesthetised, underwent electrophysiological tests and were then allowed to recover. The researchers state that one of the monkeys had been
trained (although they do not describe how) to reach, grasp and pull an object using a robotic framework designed by the researchers.
Human patients with similar spinal surgeries were also tested.
Despite the authors' assertion that
All procedures were carried out in accordance to…the principle of the 3Rs…,** essentially similar or at least clinically relevant data were obtained from people who had spinal surgery. The authors also cited numerous studies on people with spinal cord injury, studies that have resulted in considerable knowledge about spinal cord injury in people.
Thirty male long-tailed macaques were used in this research carried out to evaluate the different stages of liver fibrosis (scarring of liver tissue) in long-tailed macaques.
Eight of these individuals died before the study was completed. The work was done at The Third Affiliated Hospital of Guangxi Medical University in China, approved by the medical ethics committee and experimental animal ethics committee, and funded by various sources within China. See our news report.
The macaques were injected subcutaneously with carbon tetrachloride twice a week. Carbon tetrachloride is used as a solvent for oils and fats, as a refrigerant and as a dry-cleaning agent. It is highly toxic and carcinogenic. Presumably to add to the liver damage suffered by these monkeys, they were also fed a high-fat diet supplemented with about 35% cholesterol and the only source of fluids given to them was an ethanol (alcohol) solution (10% in water). Every four weeks after the injection with carbon tetrachloride, the macaques were anaesthetised with ketamine in order to get liver tissue via needle aspiration. The animals were also subjected to magnetic resonance imaging (MRI). After 20 weeks, the macaques were anaesthetised with ketamine and killed by injecting air into an ear vein. Liver tissue was collected for examination.
Eight of the macaques died during the study. The remaining 22, who were killed, were all found to have fibrosis of the liver, most with severe damage. The authors of the paper provide no details of the clinical condition of the monkeys, especially those individuals who died during this appalling 'experiment'.
The aim of the work was to develop a
model of liver fibrosis in the long-tailed macaque. The artificial nature of the research, the lack of application to people who suffer liver damage caused by a variety of factors (and not by injecting themselves with a solvent!) and the infliction of such severe suffering on sentient beings is morally and scientifically unacceptable. It leaves us wondering what working definition of
ethics was used by the institution's
medical ethics committee and experimental animal ethics committee.
Young monkeys were deliberately subjected to extremely cruel and barbaric treatment in an attempt to simulate human teenage depression. Although this recently published research was done at Chongqing Medical University in China and supported mostly with Chinese funding, there are two US authors, one from Wake Forest School of Medicine and another from Virginia Commonwealth University. The work was approved by the Ethics Committee of Chongqing Medical University, but there is no mention of any oversight or decisions by ethics committees at either of the US facilities. The latter is disturbing in and of itself. See our take action alert.
In the research, ten male adolescent long-tailed macaques, some less than two years old, were used. They were housed singly in cages. The 'experimental' group (as opposed to those serving as 'controls') were subjected to
chronic unpredictable mild stress for seven days, and then observed for four days. This cycle was repeated four more times. The stressors the monkeys were subjected to, in some cases lasting 24 hours, included:
Noise: A buzzer with a 100 decibel shrill chirp was placed in the monkeys' room for 12 hours from 8:00 PM to 8:00 AM the following day. According to the US Centers for Disease Control and Prevention (CDC) noise above just 70 decibels over a prolonged period of time may start to damage your hearing.
Water deprivation: The monkeys were deprived of water for 12 hours from 8:00 PM to 8:00 AM the following day.
Food deprivation: The monkeys were deprived of food for 24 hours from 8:00 AM to 8:00 AM the following day.
Space restriction: The cage space of the monkeys was restricted by a push-pull device for four hours from 8:00 AM to 12:00 PM.
Cold stress: The monkeys were sprayed with 10°C water for ten minutes. Although long-tailed macaques are known for their affinity for water, the natural conditions do not involve such cold water nor for such an extended period. Further, under natural conditions, the macaques emerge into a warm and usually sunny environment so that they can dry off quickly.
Exposure to stroboscope: Flashing stroboscopes were placed facing the monkey cages for 12 hours from 8:00 PM to 8:00 AM the following day. Flicker causes disturbance and can cause physiological effects such as headaches, at least in people.
Inescapable foot shocks: The monkeys were exposed to foot shocks by an electric shock stick from which they could not escape. The shock was 6 volts lasting 10-15 seconds with intervals of ten seconds. The monkeys received 3-4 rounds of this.
Two different stressors were used each day. The macaques' behaviour was observed and recorded, their weight was recorded and certain standard 'tests' were done to see the difference between 'controls' and stressed individuals. The researchers were looking for both depressive-like behaviours and anxiety-like behaviours, such as a huddle posture, self-clasping with head at or below the shoulders.
This is a shocking and damning indictment of the appalling way in which non-human primates are abused in the name of science. The mental anguish and torment these monkeys must have suffered is unimaginable. As a gesture to
ethics, the researchers provided the monkeys with an eight-hour window each day when they allowed the animals social contact with each other, toys (already scientifically proven to be of only transient value) and fruit and vegetables (apart from those of food deprivation)
…to meet experimental requirements set by the institutional animal care and use committee…, which they admit may have influenced the outcome of the study.
The researchers were trying to establish a non-human primate 'model' for human adolescent depression, and yet their paper states that
…youth with depression experience more serious impairments in global functioning, an increased risk of tobacco smoking and other substance abuse2. Moreover, suicide is the third leading cause of death in adolescents; and among depressed youth, 29% experience suicidal thoughts and 11% attempt suicide4. All these depressive behaviours, critical in understanding and helping troubled human adolescents, are not reproducible in non-human primates, rendering the authors' abjectly inhumane 'model' of no relevance.
The researchers stated that they were able to
…induce depressive-like and anxiety-like behaviors… in macaques. By using such terms, however, the authors clearly acknowledge that their 'model' is only superficially similar to the situation seen in adolescent human teenagers. Despite this, the researchers conclude that subjecting macaques to chronic stress provides
…a promising model to study the mechanisms underlying adolescent depression. No amount of artificial and cruel stressors inflicted on macaques can compare with the complex emotional, genetic and environmental stressors that cause mental illness and depression in human teenagers.
The Wake Forest School of Medicine claims that the goal of their Animal Welfare Program
…is to ensure that animals at Wake Forest are always treated ethically and humanely. Virginia Commonwealth University states that their Animal research program places
…the ethical treatment of animals as a primary responsibility and the founding principal [sic] of our animal care and use program and that they employ the
…ethical mandates, known as 'The Three Rs' of animal research (Reduction, Replacement and Refinement). Did these institutions approve the involvement of their representatives in research that was not only extremely cruel, but also of no scientific merit? Would they consider the research they endorsed, albeit indirectly, to be 'humane'?
Tragically for the common marmoset (Callithrix jacchus), researchers are looking to this species as a new non-human primate 'model' to be used in neurophysiology, for visual processing and behaviour.
This research was carried out at Monash University, Australia, approved by the Monash Animal Research Platform Animal Ethics Committee and publicly funded by the Australian Research Council and by the National Health and Medical Research Council of Australia. See our take action alert.
Five adult common marmosets were used (one female, four males). They were anaesthetised and subjected to invasive surgery: a tracheotomy (an incision into the windpipe), vein cannulation and craniotomy (opening up the skull) to implant electrodes into their brains. Once the surgical procedures were done, while still anaesthetised, the animals were given pancuronium bromide, which is a neuromuscular blocking agent that paralyses the animals, prevents breathing (they must be artificially ventilated) and potentially can allow them to feel pain without being able to show this by moving. The monkeys were artificially ventilated with a mixture of nitrous oxide (commonly known as laughing gas) and oxygen; there was no mention of any further anaesthetic. Recordings were made while visual stimuli were presented to the paralysed animals. The recordings went on for 2-3 days, after which the animals were killed by being given a lethal dose of sodium pentobarbital.
In addition to the inhumanity of keeping marmosets in captivity and subjecting them to research, we are concerned that, following painful surgery, the marmosets were paralysed and kept alive for up to three days. Nitrous oxide was the only anaesthetic used during this period, but it is a very weak general anaesthetic that might not have been sufficient to render the marmosets insensitive not only to the damage done by the surgeries, but also to the serious problems cause by being paralysed. It is well known that people who receive neuromuscular blocking agents for medical reasons experience a visceral reaction of extreme fear and distress even though they are being artificially respirated. They, of course, would understand that they were in no harm, something not possible in the marmosets under similar circumstances. Although it was alleged that the marmosets were
continuously monitored, because they were kept paralysed, there is no unequivocal way of knowing they did not experience fear or pain.
This experiment was basic research with no particular human benefit. The authors clearly want to continue using the marmoset as a 'model' for human brain function, as evidenced by their statement:
The marmoset offers exciting new opportunities to study links between brain physiology and behavior, but the functions of frontal cortex areas are still being identified in this species. Here, we provide the first evidence of visual receptive fields in the marmoset dorsolateral frontal cortex, an important step towards future studies of visual cognitive behavior. This does not bode well for marmosets at Monash University.
You can find out more information on all grants made by the National Institutes of Health, the largest funding entity in the US, and all their agencies by using the NIH RePORTER. You can search by grant number, investigator, recipient institution and more (see instructions).